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Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Jurkat cells, A172 cells, A549 cells, Primary antibody: CSB-PA014708(SMC2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Jurkat cells, A172 cells, A549 cells, Primary antibody: CSB-PA014708(SMC2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Jurkat cells, A172 cells, A549 cells, Primary antibody: CSB-PA014708(SMC2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds

SMC2 Antibody

CSB-PA014708
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSMC2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SMC2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10592
  • Target name
    SMC2
  • Target description
    structural maintenance of chromosomes 2
  • Target synonyms
    CAPE; CAP-E; chromosome-associated protein E; SMC protein 2; SMC-2; SMC2 (structural maintenance of chromosomes 2, yeast)-like 1; SMC2L1; structural maintenance of chromosomes (SMC) family member, chromosome-associated protein E; structural maintenance of chromosomes protein 2; XCAP-E homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95347
  • Protein Name
    Structural maintenance of chromosomes protein 2
  • Scientific Description
    Members of the structural maintenance of chromosomes, or SMC, family (e.g., SMC1A; MIM 300040) are critical for mitotic chromosome condensation in frogs and for DNA repair in mammals.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMC2 using anti-SMC2 antibody (A04804-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC2 antigen affinity purified polyclonal antibody (Catalog # A04804-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC2 at approximately 136 kDa. The expected band size for SMC2 is at 136 kDa.
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