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SMC4 antibody

GTX17958
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
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Product group Antibodies
TargetSMC4
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Overview

  • Supplier
    GeneTex
  • Product Name
    SMC4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000 - 1:10000. ICC/IF: 1:200 - 1:400. IHC-P: 1:200 - 1:400. IP: 2 - 10 microg/mg lysate. PLA: 1:1000 - 1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10051
  • Target name
    SMC4
  • Target description
    structural maintenance of chromosomes 4
  • Target synonyms
    CAPC; CAP-C; chromosome-associated polypeptide C; SMC protein 4; SMC-4; SMC4 structural maintenance of chromosomes 4-like 1; SMC4L1; structural maintenance of chromosomes protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NTJ3
  • Protein Name
    Structural maintenance of chromosomes protein 4
  • Scientific Description
    Members of the structural maintenance of chromosomes, or SMC, family (e.g., SMC1A; MIM 300040) are critical for mitotic chromosome condensation in frogs and for DNA repair in mammals.[supplied by OMIM]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 3. Flow Cytometry analysis of HL-60 cells using anti-SMC4 antibody (A04887-1). Overlay histogram showing HL-60 cells stained with A04887-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMC4 Antibody (A04887-1, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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