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SMCR8 antibody [HL1007]

GTX635798
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSMCR8
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Overview

  • Supplier
    GeneTex
  • Product Name
    SMCR8 antibody [HL1007]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1007
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID140775
  • Target name
    SMCR8
  • Target description
    SMCR8-C9orf72 complex subunit
  • Target synonyms
    DENND8A, guanine nucleotide exchange protein SMCR8, Smith-Magenis syndrome chromosome region, candidate 8, smith-Magenis syndrome chromosomal region candidate gene 8 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TEV9
  • Protein Name
    Guanine nucleotide exchange protein SMCR8
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Bauer CS, Cohen RN, Sironi F, et al. An interaction between synapsin and C9orf72 regulates excitatory synapses and is impaired in ALS/FTD. Acta Neuropathol. 2022,144(3):437-464. doi: 10.1007/s00401-022-02470-z
    Read this paper

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Figure 1. Western blot analysis of SMCR8 using anti-SMCR8 antibody (A14158-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMCR8 antigen affinity purified polyclonal antibody (Catalog # A14158-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMCR8 at approximately 140 kDa. The expected band size for SMCR8 is at 105 kDa.
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