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Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SMOC1(GTX119208) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SMOC1(GTX119208) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SMOC1(GTX119208) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

SMOC1 antibody [N2C3]

GTX119208
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSMOC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SMOC1 antibody [N2C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.05 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64093
  • Target name
    SMOC1
  • Target description
    SPARC related modular calcium binding 1
  • Target synonyms
    OAS, SPARC-related modular calcium-binding protein 1, secreted modular calcium-binding protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H4F8
  • Protein Name
    SPARC-related modular calcium-binding protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMOC1 using anti-SMOC1 antibody (A07736). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMOC1 antigen affinity purified polyclonal antibody (Catalog # A07736) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMOC1 at approximately 70 kDa. The expected band size for SMOC1 is at 48 kDa.
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