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ProductsBack/SMOX / PAO Antibody (Biotin)

SMOX / PAO Antibody (Biotin)

LS-C400115
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityMouse
TargetSMOX
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SMOX / PAO Antibody (Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID54498
  • Target name
    SMOX
  • Target description
    spermine oxidase
  • Target synonyms
    C20orf16, PAO, PAO-1, PAO1, PAOH, PAOH1, SMO, spermine oxidase, flavin containing amine oxidase, flavin-containing spermine oxidase, polyamine oxidase 1, putative cyclin G1 interacting protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMOX using anti-SMOX antibody (A05519-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMOX antigen affinity purified polyclonal antibody (Catalog # A05519-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMOX at approximately 69 kDa. The expected band size for SMOX is at 62 kDa.
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