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ProductsBack/SMS / Spermine Synthase Antibody (clone OTI 5F9)

SMS / Spermine Synthase Antibody (clone OTI 5F9)

LS-C173074
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
TargetSMS
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SMS / Spermine Synthase Antibody (clone OTI 5F9)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    Flo (1:100), IF (1:100), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI 5F9
  • Concentration
    0.36 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6611
  • Target name
    SMS
  • Target description
    spermine synthase
  • Target synonyms
    MRSR; MRXSSR; spermidine aminopropyltransferase; spermine synthase; SPMSY; SpS; SRS
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Spermine Synthase/SMS using anti-Spermine Synthase/SMS antibody (A01831-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spermine Synthase/SMS antigen affinity purified polyclonal antibody (Catalog # A01831-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spermine Synthase/SMS at approximately 41 kDa. The expected band size for Spermine Synthase/SMS is at 41 kDa.
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