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SNAI1 antibody

GTX100754
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSNAI1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SNAI1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.94 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6615
  • Target name
    SNAI1
  • Target description
    snail family transcriptional repressor 1
  • Target synonyms
    SLUGH2, SNA, SNAH, SNAIL, SNAIL1, dJ710H13.1, zinc finger protein SNAI1, protein sna, protein snail homolog 1, snail 1 homolog, snail 1 zinc finger protein, snail family zinc finger 1, snail homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95863
  • Protein Name
    Zinc finger protein SNAI1
  • Scientific Description
    The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wang CW, Chiou HC, Chen SC, et al. Arsenic exposure and lung fibrotic changes-evidence from a longitudinal cohort study and experimental models. Front Immunol. 2023,14:1225348. doi: 10.3389/fimmu.2023.1225348
    Read this paper
  • Chiou HC, Wang CW, Chen SC, et al. Copper Exposure Induces Epithelial-Mesenchymal Transition-Related Fibrotic Change via Autophagy and Increase Risk of Lung Fibrosis in Human. Antioxidants (Basel). 2023,12(2). doi: 10.3390/antiox12020532
    Read this paper
  • Kano H, Izumi K, Hiratsuka K, et al. Suppression of androgen receptor signaling induces prostate cancer migration via activation of the CCL20-CCR6 axis. Cancer Sci. 2023,114(4):1479-1490. doi: 10.1111/cas.15683
    Read this paper
  • Chen YQ, Hung CY, Wei MT, et al. Snail Augments Nuclear Deformability to Promote Lymph Node Metastasis of Head and Neck Squamous Cell Carcinoma. Front Cell Dev Biol. 2022,10:809738. doi: 10.3389/fcell.2022.809738
    Read this paper
  • Brzozowa-Zasada M. Immunohistochemical expression of Snail1 protein in colorectal adenocarcinoma samples and its prognostic activity in Caucasian patients. Prz Gastroenterol. 2021,16(4):339-345. doi: 10.5114/pg.2021.111765
    Read this paper
  • Xiao L, Zhang C, Li X, et al. LEF1 Enhances the Progression of Colonic Adenocarcinoma via Remodeling the Cell Motility Associated Structures. Int J Mol Sci. 2021,22(19). doi: 10.3390/ijms221910870
    Read this paper
  • Chang YX, Lin YF, Chen CL, et al. Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways. Cancers (Basel). 2020,12(12). doi: 10.3390/cancers12123865
    Read this paper
  • Chung HH, Lee CT, Hu JM, et al. NKX6.1 Represses Tumorigenesis, Metastasis, and Chemoresistance in Colorectal Cancer. Int J Mol Sci. 2020,21(14). doi: 10.3390/ijms21145106
    Read this paper
  • Xiao L, Jin H, Duan W, et al. Roles of N-terminal Annexin A2 phosphorylation sites and miR-206 in colonic adenocarcinoma. Life Sci. 2020,253:117740. doi: 10.1016/j.lfs.2020.117740
    Read this paper
  • Li JM, Tseng CW, Lin CC, et al. Upregulation of LGALS1 is associated with oral cancer metastasis. Ther Adv Med Oncol. 2018,10:1758835918794622. doi: 10.1177/1758835918794622
    Read this paper

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Figure 1. Western blot analysis of SNAIL/SNAI1 using anti-SNAIL/SNAI1 antibody (A00716-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human SW620 whole cell lysates, Lane 8: human Raji whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAIL/SNAI1 antigen affinity purified polyclonal antibody (Catalog # A00716-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAIL/SNAI1 at approximately 29 kDa. The expected band size for SNAIL/SNAI1 is at 29 kDa.
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