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SNAI3 Antibody (aa155-178)

LS-C407653
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSNAI3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SNAI3 Antibody (aa155-178)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID333929
  • Target name
    SNAI3
  • Target description
    snail family transcriptional repressor 3
  • Target synonyms
    SMUC, SNAIL3, ZNF293, Zfp293, zinc finger protein SNAI3, protein snail homolog 3, snail family zinc finger 3, snail homolog 3, zinc finger protein 293
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNAI3 using anti-SNAI3 antibody (PB9440). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI3 antigen affinity purified polyclonal antibody (Catalog # PB9440) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI3 at approximately 32 kDa. The expected band size for SNAI3 is at 32 kDa.
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