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Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA365029 (SNAI3 Antibody) at dilution 1/20 (Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA365029 (SNAI3 Antibody) at dilution 1/20 (Original magnification: x200)
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA365029 (SNAI3 Antibody) at dilution 1/20 (Original magnification: x200)

SNAI3 Rabbit Polyclonal Antibody

TA365029
OriGene
ApplicationsImmunoHistoChemistry
DownloadMSDS
Product group Antibodies
TargetSNAI3
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SNAI3 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID333929
  • Target name
    SNAI3
  • Target description
    snail family transcriptional repressor 3
  • Target synonyms
    protein snail homolog 3; SMUC; snail family zinc finger 3; snail homolog 3; SNAIL3; Zfp293; zinc finger protein 293; zinc finger protein SNAI3; ZNF293
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ3KNW1
  • Protein Name
    Zinc finger protein SNAI3
  • Scientific Description
    SNAI3 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA558561(SNAI3 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of SNAI3 using anti-SNAI3 antibody (PB9440). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAI3 antigen affinity purified polyclonal antibody (Catalog # PB9440) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAI3 at approximately 32 kDa. The expected band size for SNAI3 is at 32 kDa.
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