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Western Blot analysis of HT-29 cell using SNCG Polyclonal Antibody at dilution of 1:800
Western Blot analysis of HT-29 cell using SNCG Polyclonal Antibody at dilution of 1:800
Western Blot analysis of HT-29 cell using SNCG Polyclonal Antibody at dilution of 1:800

SNCG Polyclonal Antibody

E-AB-10081
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSNCG
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Overview

  • Supplier
    Elabscience
  • Product Name
    SNCG Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6623
  • Target name
    SNCG
  • Target description
    synuclein gamma
  • Target synonyms
    BCSG1, SR, gamma-synuclein, -synuclein, E pound-synuclein, breast cancer-specific gene 1 protein, persyn, synoretin, synuclein, gamma (breast cancer-specific protein 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO76070
  • Protein Name
    Gamma-synuclein
  • Scientific Description
    This gene encodes a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. Mutations in this gene have also been associated with breast tumor development.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of gamma Synuclein/SNCG using anti-gamma Synuclein/SNCG antibody (A03523-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-gamma Synuclein/SNCG antigen affinity purified polyclonal antibody (Catalog # A03523-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for gamma Synuclein/SNCG at approximately 17 kDa. The expected band size for gamma Synuclein/SNCG is at 13 kDa.
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Wild-type (WT) and gamma Synuclein knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with gamma Synuclein antibody (GTX110483) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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