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SNRNP200 Antibody (aa1-50)

LS-C290126
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSNRNP200
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SNRNP200 Antibody (aa1-50)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:100 - 1:500), IP (2 - 10 µg/mg lysate), WB (1:2000 - 1:10000) IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23020
  • Target name
    SNRNP200
  • Target description
    small nuclear ribonucleoprotein U5 subunit 200
  • Target synonyms
    activating signal cointegrator 1 complex subunit 3-like 1; ASCC3L1; bad response to refrigeration 2 homolog; BRR2; BRR2 homolog; HELIC2; RP33; small nuclear ribonucleoprotein 200kDa (U5); small nuclear ribonucleoprotein, U5 200kDa subunit; U5 small nuclear ribonucleoprotein 200 kDa helicase; U5 snRNP-specific 200 kDa protein; U5-200KD
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNRNP200 using anti-SNRNP200 antibody (A04514-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRNP200 antigen affinity purified polyclonal antibody (Catalog # A04514-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRNP200 at approximately 245 kDa. The expected band size for SNRNP200 is at 245 kDa.
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