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SNRNP40 Antibody

CSB-PA822196LA01HU
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetSNRNP40
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Overview

  • Supplier
    Cusabio
  • Product Name
    SNRNP40 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9410
  • Target name
    SNRNP40
  • Target description
    small nuclear ribonucleoprotein U5 subunit 40
  • Target synonyms
    40K, HPRP8BP, PRP8BP, PRPF8BP, SPF38, WDR57, U5 small nuclear ribonucleoprotein 40 kDa protein, 38 kDa-splicing factor, Prp8-binding protein, U5 snRNP 40 kDa protein, U5 snRNP-specific 40 kDa protein, U5-40K, U5-40kD protein, WD repeat domain 57 (U5 snRNP specific), WD repeat-containing protein 57, small nuclear ribonucleoprotein 40kDa (U5), small nuclear ribonucleoprotein, U5 40kDa subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DI7
  • Protein Name
    U5 small nuclear ribonucleoprotein 40 kDa protein
  • Scientific Description
    Component of the U5 small nuclear ribonucleoprotein (snRNP) complex. The U5 snRNP is part of the spliceosome, a multiprotein complex that catalyzes the removal of introns from pre-messenger RNAs.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRNP40 antigen affinity purified polyclonal antibody (Catalog # A12991-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRNP40 at approximately 37 kDa. The expected band size for SNRNP40 is at 39 kDa.
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