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SNRNP70 Antibody

CSB-PA714018ZA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSNRNP70
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Overview

  • Supplier
    Cusabio
  • Product Name
    SNRNP70 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6625
  • Target name
    SNRNP70
  • Target description
    small nuclear ribonucleoprotein U1 subunit 70
  • Target synonyms
    RNPU1Z; RPU1; small nuclear ribonucleoprotein 70kDa (U1); small nuclear ribonucleoprotein, U1 70kDa subunit; Snp1; SNRP70; U1 small nuclear ribonucleoprotein 70 kDa; U1 snRNP 70 kDa; U170K; U1-70K; U1AP; U1RNP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08621
  • Protein Name
    U1 small nuclear ribonucleoprotein 70 kDa
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNRNP70 using anti-SNRNP70 antibody (A03527-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRNP70 antigen affinity purified polyclonal antibody (Catalog # A03527-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRNP70 at approximately 95 kDa. The expected band size for SNRNP70 is at 52 kDa.
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