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SNRPA1 Rabbit mAb

A0647
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSNRPA1
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Overview

  • Supplier
    ABclonal
  • Product Name
    SNRPA1 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID6627
  • Target name
    SNRPA1
  • Target description
    small nuclear ribonucleoprotein polypeptide A'
  • Target synonyms
    Lea1, U2A', U2 small nuclear ribonucleoprotein A', U2 small nuclear ribonucleoprotein polypeptide A', U2 snRNP A'
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09661
  • Protein Name
    U2 small nuclear ribonucleoprotein A'
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPA1 antigen affinity purified polyclonal antibody (Catalog # A10655-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRPA1 at approximately 28 kDa. The expected band size for SNRPA1 is at 28 kDa.
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