WB analysis of Syntrophin knockdown U87MG cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX11425 SNTB2 antibody [1351]. Dilution : 0.1-1 microg/ml
![WB analysis of canine lysate using GTX11425 SNTB2 antibody [1351].](https://www.genetex.com/upload/website/prouct_img/normal/GTX11425/GTX11425_1471_WB_w_23060501_354.webp "WB analysis of canine lysate using GTX11425 SNTB2 antibody [1351].")
![WB analysis of whole cell extracts (30 microg lysate) of U-87MG (Lane 1), PC 3 (Lane 2) and HeLa (Lane 3) using GTX11425 SNTB2 antibody [1351]. Dilution : 0.1-1 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX11425/GTX11425_1472_WB_w_23060501_485.webp "WB analysis of whole cell extracts (30 microg lysate) of U-87MG (Lane 1), PC 3 (Lane 2) and HeLa (Lane 3) using GTX11425 SNTB2 antibody [1351]. Dilution : 0.1-1 microg/ml")
![ICC/IF analysis of PC-3 cells using GTX11425 SNTB2 antibody [1351]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX11425/GTX11425_234_ICC-IF_w_23060501_235.webp "ICC/IF analysis of PC-3 cells using GTX11425 SNTB2 antibody [1351]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Trito X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature")
![ICC/IF analysis of 293 cells using GTX11425 SNTB2 antibody [1351]. Cells were probed without (right) or with (left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX11425/GTX11425_235_ICC-IF_w_23060501_139.webp "ICC/IF analysis of 293 cells using GTX11425 SNTB2 antibody [1351]. Cells were probed without (right) or with (left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of A2058 Cells using GTX11425 SNTB2 antibody [1351]. Cells were probed without (right) or with (left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX11425/GTX11425_236_ICC-IF_w_23060501_292.webp "ICC/IF analysis of A2058 Cells using GTX11425 SNTB2 antibody [1351]. Cells were probed without (right) or with (left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX11425 SNTB2 antibody [1351]. Cells were probed without (right) or with (left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX11425/GTX11425_237_ICC-IF_w_23060501_127.webp "ICC/IF analysis of HeLa cells using GTX11425 SNTB2 antibody [1351]. Cells were probed without (right) or with (left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
WB analysis of Syntrophin knockdown U87MG cells (lane 3), scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1) using GTX11425 SNTB2 antibody [1351]. Dilution : 0.1-1 microg/ml
SNTB2 antibody [1351]
GTX11425
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityAmphibian, Canine, Chicken, Human, Mouse, Rat
TargetSNTB2
Overview
- SupplierGeneTex
- Product NameSNTB2 antibody [1351] - KO/KD Validated
- Delivery Days Customer9
- Antibody SpecificityThis antibody detects alpha 1, beta 1 and beta 2 subunits of syntrophin. Characterization studies of this antibody have demonstrated that clone 1351 is directed against an epitope within the PDZ domain of syntrophin.
- Application Supplier NoteWB: 0.1-1 microg/ml. ICC/IF: 1-2 microg/ml. FACS: 0.5microg/106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1351
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6645
- Target nameSNTB2
- Target descriptionsyntrophin beta 2
- Target synonyms59 kDa dystrophin-associated protein A1 basic component 2; beta-2-syntrophin; D16S2531E; dystrophin-associated protein A1, 59kD, basic component 2; EST25263; SNT2B2; SNT3; SNTL; syntrophin, beta 2 (dystrophin-associated protein A1, 59kDa, basic component 2); syntrophin-3
- HostMouse
- IsotypeIgG1
- Protein IDQ13425
- Protein NameBeta-2-syntrophin
- Scientific DescriptionDystrophin is a large, rod-like cytoskeletal protein found at the inner surface of muscle fibers. Dystrophin is missing in Duchenne Muscular Dystrophy patients and is present in reduced amounts in Becker Muscular Dystrophy patients. The protein encoded by this gene is a peripheral membrane protein found associated with dystrophin and dystrophin-related proteins. This gene is a member of the syntrophin gene family, which contains at least two other structurally-related genes. [provided by RefSeq, Jul 2008]
- ReactivityAmphibian, Canine, Chicken, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203