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Western Blot analysis of K562 cells using SNX1 Polyclonal Antibody
Western Blot analysis of K562 cells using SNX1 Polyclonal Antibody
Western Blot analysis of K562 cells using SNX1 Polyclonal Antibody

SNX1 Antibody

CSB-PA004128
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSNX1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SNX1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6642
  • Target name
    SNX1
  • Target description
    sorting nexin 1
  • Target synonyms
    HsT17379, VPS5, sorting nexin-1, sorting nexin 1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13596
  • Protein Name
    Sorting nexin-1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of SNX1 using anti-SNX1 antibody (A02692-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX1 antigen affinity purified polyclonal antibody (Catalog # A02692-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX1 at approximately 70-75 kDa. The expected band size for SNX1 is at 59 kDa.
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