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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA239255(SNX30 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA239255(SNX30 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA239255(SNX30 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

SNX30 Antibody

CSB-PA239255
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSNX30
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Overview

  • Supplier
    Cusabio
  • Product Name
    SNX30 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID401548
  • Target name
    SNX30
  • Target description
    sorting nexin family member 30
  • Target synonyms
    ATG24A, sorting nexin-30
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VWJ9
  • Protein Name
    Sorting nexin-30
  • Scientific Description
    May be involved in several stages of intracellular trafficking.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SNX30 using anti-SNX30 antibody (A14226-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX30 antigen affinity purified polyclonal antibody (Catalog # A14226-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX30 at approximately 50 kDa. The expected band size for SNX30 is at 50 kDa.
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Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
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