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Western blot All lanes: SOCS4 antibody at 4.39 ug/ml Lane 1: Mouse kidney tissue Lane 2: Jurkat whole cell lysate Lane 3: A549 whole cell lysate Lane 4: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa
Western blot All lanes: SOCS4 antibody at 4.39 ug/ml Lane 1: Mouse kidney tissue Lane 2: Jurkat whole cell lysate Lane 3: A549 whole cell lysate Lane 4: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa
Western blot All lanes: SOCS4 antibody at 4.39 ug/ml Lane 1: Mouse kidney tissue Lane 2: Jurkat whole cell lysate Lane 3: A549 whole cell lysate Lane 4: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa

SOCS4 Antibody

CSB-PA022393ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSOCS4
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Overview

  • Supplier
    Cusabio
  • Product Name
    SOCS4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID122809
  • Target name
    SOCS4
  • Target description
    suppressor of cytokine signaling 4
  • Target synonyms
    SH2 domain containing SOCS box protein; SOCS-4; SOCS7; SOCS-7; suppressor of cytokine signaling 4; suppressor of cytokine signaling 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WXH5
  • Protein Name
    Suppressor of cytokine signaling 4
  • Scientific Description
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. Substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits EGF signaling by mediating the degradation of the Tyr-phosphorylated EGF receptor/EGFR.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOCS4 using anti-SOCS4 antibody (PB9506). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug, Lane 2: SW620 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: SKOV Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS4 antigen affinity purified polyclonal antibody (Catalog # PB9506) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS4 at approximately 51 kDa. The expected band size for SOCS4 is at 51 kDa.
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