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SOD1 antibody

GTX100659
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
DownloadDatasheet
Product group Antibodies
TargetSOD1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SOD1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.21 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6647
  • Target name
    SOD1
  • Target description
    superoxide dismutase 1
  • Target synonyms
    ALS; ALS1; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HEL-S-44; homodimer; hSod1; indophenoloxidase A; IPOA; SOD; SOD, soluble; STAHP; superoxide dismutase [Cu-Zn]; superoxide dismutase 1, soluble; superoxide dismutase, cystolic
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00441
  • Protein Name
    Superoxide dismutase [Cu-Zn]
  • Scientific Description
    The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence.
    Read more
  • CCL5 via GPX1 activation protects hippocampal memory function after mild traumatic brain injury. Ho MH et al., 2021 Oct, Redox Biol
    Read more
  • Hydrogen peroxide toxicity on auditory cells: An in vitro study. Gentilin E et al., 2021 Aug 25, Chem Biol Interact
    Read more
  • Melatonin Successfully Rescues the Hippocampal Molecular Machinery and Enhances Anti-oxidative Activity Following Early-Life Sleep Deprivation Injury. Chang HM et al., 2021 May 13, Antioxidants (Basel)
    Read more
  • Oral administration of Jumihaidokuto inhibits UVB-induced skin damage and prostaglandin E2 production in HR-1 hairless mice. Murata K et al., 2021 Jan, J Nat Med
    Read more
  • Aldehyde dehydrogenase 2 protects against abdominal aortic aneurysm formation by reducing reactive oxygen species, vascular inflammation, and apoptosis of vascular smooth muscle cells. Tsai SH et al., 2020 Jul, FASEB J
    Read more
  • The Phenoxyphenol Compound 4-HPPP Selectively Induces Antiproliferation Effects and Apoptosis in Human Lung Cancer Cells through Aneupolyploidization and ATR DNA Repair Signaling. Liu W et al., 2020, Oxid Med Cell Longev
    Read more
  • Reduced camptothecin sensitivity of estrogen receptor-positive human breast cancer cells following exposure to di(2-ethylhexyl)phthalate (DEHP) is associated with DNA methylation changes. Chou CK et al., 2019 Apr, Environ Toxicol
    Read more
  • Exogenous C8-Ceramide Induces Apoptosis by Overproduction of ROS and the Switch of Superoxide Dismutases SOD1 to SOD2 in Human Lung Cancer Cells. Chang YC et al., 2018 Oct 2, Int J Mol Sci
    Read more
  • Bioavailability of andrographolide and protection against carbon tetrachloride-induced oxidative damage in rats. Chen HW et al., 2014 Oct 1, Toxicol Appl Pharmacol
    Read more

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Figure 1. Western blot analysis of SOD1 using anti-SOD1 antibody (PB9402). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD1 antigen affinity purified polyclonal antibody (Catalog # PB9402) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD1 at approximately 16-18 kDa. The expected band size for SOD1 is at 16 kDa.
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