![ICC/IF analysis of PANC-1 (left) and SKBR-3 (right) cells using GTX83012 SOD1 antibody [6F5]. Green : SOD1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments ICC/IF analysis of PANC-1 (left) and SKBR-3 (right) cells using GTX83012 SOD1 antibody [6F5]. Green : SOD1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX83012/GTX83012_20170912_ICCIF_1_w_23061322_886.webp)
ICC/IF analysis of PANC-1 (left) and SKBR-3 (right) cells using GTX83012 SOD1 antibody [6F5]. Green : SOD1 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
SOD1 antibody [6F5]
GTX83012
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSOD1
Overview
- SupplierGeneTex
- Product NameSOD1 antibody [6F5]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6F5
- ConjugateUnconjugated
- Gene ID6647
- Target nameSOD1
- Target descriptionsuperoxide dismutase 1
- Target synonymsALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer, superoxide dismutase [Cu-Zn], Cu/Zn superoxide dismutase, SOD, soluble, epididymis secretory protein Li 44, indophenoloxidase A, superoxide dismutase 1, soluble, superoxide dismutase, cystolic
- HostMouse
- IsotypeIgG1
- Protein IDP00441
- Protein NameSuperoxide dismutase [Cu-Zn]
- Scientific DescriptionThe protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Hathaway CK, Grant R, Hagaman JR, et al. Endothelin-1 critically influences cardiac function via superoxide-MMP9 cascade. Proc Natl Acad Sci U S A. 2015,112(16):5141-6. doi: 10.1073/pnas.1504557112Read this paper
![FACS analysis of A431 cells using GTX83012 SOD1 antibody [6F5]. Green : SOD1 Purple : negative control FACS analysis of A431 cells using GTX83012 SOD1 antibody [6F5]. Green : SOD1 Purple : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX83012/GTX83012_20170912_FACS_w_23061322_639.webp)
![WB analysis of HeLa (1), NIH3T3 (2), A549 (3) and A431 (4) cell lysate using GTX83012 SOD1 antibody [6F5]. WB analysis of HeLa (1), NIH3T3 (2), A549 (3) and A431 (4) cell lysate using GTX83012 SOD1 antibody [6F5].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83012/GTX83012_20170912_WB_w_23061322_882.webp)
![ICC/IF analysis of 3T3-L1 cells using GTX83012 SOD1 antibody [6F5]. Green : SOD1 Blue: DRAQ5 fluorescent DNA dye ICC/IF analysis of 3T3-L1 cells using GTX83012 SOD1 antibody [6F5]. Green : SOD1 Blue: DRAQ5 fluorescent DNA dye](https://www.genetex.com/upload/website/prouct_img/normal/GTX83012/GTX83012_20170912_ICCIF_w_23061322_522.webp)


![ICC/IF analysis of Flag-tagged SOD1 wild type or G93A mutant-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27] or MS785/MS27 cocktail. Fixation : 4% PFA for 10 min at RT.
Permeabilization : 0.2% Triton X-100 for 5 min
Dilution : 1μg/ml (12 hours at 4oC)](https://www.genetex.com/upload/website/prouct_img/normal/GTX57211/GTX57211_GTX57211_ICC_IF_1-1_w_23061123_271.webp)
![IP analysis of SOD1 wild type or mutants-expressing HEK293 cells using GTX57211 SOD1 (mutant) antibody [MS785], GTX57212 SOD1 (mutant) antibody [MS27], or MS785/MS27 cocktail. Neither MS785 nor MS27 single detected some specific mutants which have the mutation on the antibody’s epitope. MS785/MS27 cocktail overcame this problem. Validated SOD1 mutants were listed in “Appendix”.
Antibody amount : MS27 (GTX57212): 2μg, MS785 (GTX57211): 5μg, or MS27/MS785 cocktail: 1μg
IP lysis buffer: 1% Triton X100/TBS buffer.](https://www.genetex.com/upload/website/prouct_img/normal/GTX57212/GTX57212_IP_1-1_w_23061123_188.webp)

![SOD1 antibody [HL1652] detects SOD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. SOD1 stained by SOD1 antibody [HL1652] (GTX637250) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637250/GTX637250_T-44753_20220805_IHC-P_R_22080923_144.webp)
![Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL1653] (GTX637251) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637251/GTX637251_T-44753_20220729_WB_M_R_22080119_341.webp)
![SOD1 antibody [HL2033] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. SOD1 stained by SOD1 antibody [HL2033] (GTX637934) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637934/GTX637934_T-44879_20230106_IHC-P_M_23010400_601.webp)
![WB analysis of various cell lines using GTX83592 SOD1 antibody [8B10]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83592/GTX83592_3436_WB_w_23061420_872.webp)