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Sodium/Glucose Cotransporter 2 (SGLT2) Polyclonal Antibody

CAU32782
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetSLC5A2
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Overview

  • Supplier
    Biomatik
  • Product Name
    Sodium/Glucose Cotransporter 2 (SGLT2) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.44 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6524
  • Target name
    SLC5A2
  • Target description
    solute carrier family 5 member 2
  • Target synonyms
    SGLT2, sodium/glucose cotransporter 2, Na(+)/glucose cotransporter 2, low affinity sodium-glucose cotransporter, solute carrier family 5 (sodium/glucose cotransporter), member 2, solute carrier family 5 (sodium/glucose transporter), member 2
  • Host
    Rabbit
  • Protein IDP31639
  • Protein Name
    Sodium/glucose cotransporter 2
  • Scientific Description
    The Sodium/Glucose Cotransporter 2 (SGLT2) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC5A2 using anti-SLC5A2 antibody (A03748-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse spleen tissue lysates, Lane 8: mouse lung tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC5A2 antigen affinity purified polyclonal antibody (Catalog # A03748-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC5A2 at approximately 73KD. The expected band size for SLC5A2 is at 73KD.
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