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ProductsBack/Sox-15 (D-8): m-IgG Fc BP-HRP Bundle

Sox-15 (D-8): m-IgG Fc BP-HRP Bundle

SC-540182
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSOX15
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Sox-15 (D-8): m-IgG Fc BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6665
  • Target name
    SOX15
  • Target description
    SRY-box transcription factor 15
  • Target synonyms
    SOX20, SOX26, SOX27, transcription factor SOX-15, SRY (sex determining region Y)-box 15, SRY (sex determining region Y)-box 20, SRY-box 15, protein SOX-15
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SOX15 using anti-SOX15 antibody (A07722-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates Lane 2: human U-87MG whole cell lysates Lane 3: human HEK293 whole cell lysates Lane 4: human K562 whole cell lysates Lane 5: human U2OS whole cell lysates Lane 6: rat thymus tissue lysates Lane 7: mouse SP20 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX15 antigen affinity purified polyclonal antibody (Catalog # A07722-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX15 at approximately 29KD. The expected band size for SOX15 is at 26KD.
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TargetSOX15
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TargetSOX15
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Immunohistochemistry analysis in human skin and liver tissues using HPA067196 antibody. Corresponding SOX15 RNA-seq data are presented for the same tissues.
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TargetSOX15
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Sox-15 (D-8)SC-166966
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SOX15 antibody detects SOX15 protein at nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SOX15 protein stained by SOX15 antibody (GTX117940) diluted at 1:1000. Blue: Hoechst 33342 staining.
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