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Sox11 Polyclonal Antibody

CAC11205
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSOX11
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Overview

  • Supplier
    Biomatik
  • Product Name
    SOX11 Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6664
  • Target name
    SOX11
  • Target description
    SRY-box transcription factor 11
  • Target synonyms
    CSS9; MRD27; SRY (sex-determining region Y)-box 11; SRY-box 11; SRY-related HMG-box gene 11; transcription factor SOX-11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35716
  • Protein Name
    Transcription factor SOX-11
  • Scientific Description
    The Sox11 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity Supplier
    Human, Rat, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOX11 using anti-SOX11 antibody (A02603-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat spleen tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: rat lung tissue lysates, Lane 8: rat liver tissue lysates, Lane 9: mouse spleen tissue lysates, Lane 10: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX11 antigen affinity purified polyclonal antibody (Catalog # A02603-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX11 at approximately 50KD. The expected band size for SOX11 is at 50KD.
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