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ProductsBack/SOX15 antibody, Internal
WB analysis of human muscle lysate using GTX88871 SOX15 antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human muscle lysate using GTX88871 SOX15 antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human muscle lysate using GTX88871 SOX15 antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer

SOX15 antibody, Internal

GTX88871
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSOX15
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Overview

  • Supplier
    GeneTex
  • Product Name
    SOX15 antibody, Internal
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.1-0.3microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6665
  • Target name
    SOX15
  • Target description
    SRY-box transcription factor 15
  • Target synonyms
    protein SOX-15; SOX20; SOX26; SOX27; SRY (sex determining region Y)-box 15; SRY (sex determining region Y)-box 20; SRY-box 15
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDO60248
  • Protein Name
    Protein SOX-15
  • Scientific Description
    This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOX15 using anti-SOX15 antibody (A07722-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates Lane 2: human U-87MG whole cell lysates Lane 3: human HEK293 whole cell lysates Lane 4: human K562 whole cell lysates Lane 5: human U2OS whole cell lysates Lane 6: rat thymus tissue lysates Lane 7: mouse SP20 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX15 antigen affinity purified polyclonal antibody (Catalog # A07722-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX15 at approximately 29KD. The expected band size for SOX15 is at 26KD.
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