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ProductsBack/SOX17 Mouse Monoclonal Antibody [Clone ID: OTI5G6]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17. Positive lysates LY411669 (100ug) and LC411669 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17. Positive lysates LY411669 (100ug) and LC411669 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17. Positive lysates LY411669 (100ug) and LC411669 (20ug) can be purchased separately from OriGene.

SOX17 Mouse Monoclonal Antibody [Clone ID: OTI5G6]

CF500282
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSOX17
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SOX17 Mouse Monoclonal Antibody [Clone ID: OTI5G6]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5G6
  • Gene ID64321
  • Target name
    SOX17
  • Target description
    SRY-box transcription factor 17
  • Target synonyms
    PPH7, VUR3, transcription factor SOX-17, SRY (sex determining region Y)-box 17, SRY-box 17, SRY-related HMG-box transcription factor SOX17
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9H6I2
  • Protein Name
    Transcription factor SOX-17
  • Scientific Description
    Carrier-free (BSA/glycerol-free) anti-Sox17 mouse monoclonal antibody, clone OTI5G6 (formerly 5G6)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SOX17 using anti-SOX17 antibody (A01665-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX17 antigen affinity purified polyclonal antibody (Catalog # A01665-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX17 at approximately 45 kDa. The expected band size for SOX17 is at 45 kDa.
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