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ProductsBack/SOX2 antibody [HL1192]
Zebrafish tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1192] (GTX636504) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Zebrafish tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1192] (GTX636504) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Zebrafish tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1192] (GTX636504) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SOX2 antibody [HL1192]

GTX636504
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Zebra Fish
TargetSOX2
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Overview

  • Supplier
    GeneTex
  • Product Name
    SOX2 antibody [HL1192]
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1192
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6657
  • Target name
    SOX2
  • Target description
    SRY-box transcription factor 2
  • Target synonyms
    ANOP3, MCOPS3, transcription factor SOX-2, SRY (sex determining region Y)-box 2, SRY-box 2, SRY-related HMG-box gene 2, sex determining region Y-box 2, transcription factor SOX2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48431
  • Protein Name
    Transcription factor SOX-2
  • Scientific Description
    This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOX2 using anti-SOX2 antibody (A00105-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 ma for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX2 antigen affinity purified poly clonal antibody (Catalog # A00105-1) at 0.5 microg/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit ING-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Luminescence detection (ECL) kit (Catalog # EK1002) with Anon 5200 system. A specific band was detected for SOX2 at approximately 36 kDa. The expected band size for SOX2 is at 34 kDa.
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