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SOX2 Rabbit pAb

A0561
ABclonal
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSOX2
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Overview

  • Supplier
    ABclonal
  • Product Name
    SOX2 Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB,IHC,IF/ICC,IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6657
  • Target name
    SOX2
  • Target description
    SRY-box transcription factor 2
  • Target synonyms
    ANOP3, MCOPS3, transcription factor SOX-2, SRY (sex determining region Y)-box 2, SRY-box 2, SRY-related HMG-box gene 2, sex determining region Y-box 2, transcription factor SOX2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48431
  • Protein Name
    Transcription factor SOX-2
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SOX2 using anti-SOX2 antibody (A00105-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 ma for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX2 antigen affinity purified poly clonal antibody (Catalog # A00105-1) at 0.5 microg/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit ING-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Luminescence detection (ECL) kit (Catalog # EK1002) with Anon 5200 system. A specific band was detected for SOX2 at approximately 36 kDa. The expected band size for SOX2 is at 34 kDa.
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