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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA022431LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA022431LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA022431LA01HU at dilution of 1:100

SOX4 Antibody

CSB-PA022431LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSOX4
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Overview

  • Supplier
    Cusabio
  • Product Name
    SOX4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6659
  • Target name
    SOX4
  • Target description
    SRY-box transcription factor 4
  • Target synonyms
    CSS10, EVI16, IDDSDF, transcription factor SOX-4, SRY (sex determining region Y)-box 4, SRY-box 4, SRY-related HMG-box gene 4, ecotropic viral integration site 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06945
  • Protein Name
    Transcription factor SOX-4
  • Scientific Description
    Transcriptional activator that binds with high affinity to the T-cell enhancer motif 5-AACAAAG-3 motif.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SOX4 using anti-SOX4 antibody (PB9618). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate, Lane 2: 293T Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX4 antigen affinity purified polyclonal antibody (Catalog # PB9618) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX4 at approximately 47KD. The expected band size for SOX4 is at 47KD.
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