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SOX6 Polyclonal Antibody

BS-11599R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
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Overview

  • Supplier
    Bioss
  • Product Name
    SOX6 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of SOX6 using anti-SOX6 antibody (A02877-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX6 antigen affinity purified polyclonal antibody (Catalog # A02877-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX6 approximately 92KD. The expected band size for SOX6 is at 92KD.
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Western blot All lanes: SOX6 antibody at 10ug/ml Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 92, 90, 89 kDa Observed band size: 92 kDa
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Immunohistochemistry analysis in human testis and endometrium tissues using Anti-SOX6 antibody. Corresponding SOX6 RNA-seq data are presented for the same tissues.
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SOX6 antibody [N1C3] immunoprecipitates SOX6 protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 5 μg of preimmune Rabbit IgG B. Immunoprecipitation of SOX6 protein by 5 μg SOX6 antibody [N1C3] (GTX116081) 7.5 % SDS-PAGE The immunoprecipitated SOX6 protein was detected by SOX6 antibody [N1C3] (GTX116081) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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