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ProductsBack/SFTPD Polyclonal Antibody
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-SP-D/PSPD Polyclonal Antibody, Unconjugated(bs-1538R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-SP-D/PSPD Polyclonal Antibody, Unconjugated(bs-1538R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-SP-D/PSPD Polyclonal Antibody, Unconjugated(bs-1538R) at 1:200, followed by conjugation to the secondary antibody and DAB staining.

SFTPD Polyclonal Antibody

BS-1583R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSFTPD
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    SP-D/PSPD Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6441
  • Target name
    SFTPD
  • Target description
    surfactant protein D
  • Target synonyms
    COLEC7; collectin-7; lung surfactant protein D; PSP-D; pulmonary surfactant apoprotein; pulmonary surfactant-associated protein D; SFTP4; SP-D; surfactant-associated protein, pulmonary 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35247
  • Protein Name
    Pulmonary surfactant-associated protein D
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Autophagy proteins are required for club cell structure and function in airways. Malvin NP et al., 2019 Aug 1, Am J Physiol Lung Cell Mol Physiol
    Read more
  • Functional roles of C/EBPalpha and SUMO-modification in lung development. Chen YD et al., 2017 Oct, Int J Mol Med
    Read more
  • Databases for technical aspects of immunohistochemistry. Furukawa S et al., 2017 Jan, J Toxicol Pathol
    Read more
  • Autoantibody-Mediated Pulmonary Alveolar Proteinosis in Rasgrp1-Deficient Mice. Ferretti A et al., 2016 Jul 15, J Immunol
    Read more

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Figure 1. Western blot analysis of Surfactant protein D using anti-Surfactant protein D antibody (PB9617). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: PANC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Surfactant protein D antigen affinity purified polyclonal antibody (Catalog # PB9617) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Surfactant protein D at approximately 38 kDa. The expected band size for Surfactant protein D is at 38 kDa.
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