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ProductsBack/SP6 Antibody - middle region (OAAB09304)

SP6 Antibody - middle region (OAAB09304)

OAAB09304
Aviva Systems Biology
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSP6
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    SP6 Antibody - middle region (OAAB09304)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Gene ID80320
  • Target name
    SP6
  • Target description
    Sp6 transcription factor
  • Target synonyms
    AI1K, EPFN, EPIPROFIN, KLF14, transcription factor Sp6, Kruppel-like factor 14, krueppel-like factor 14
  • Host
    Rabbit
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SP6 using anti-SP6 antibody (A08730-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP6 antigen affinity purified polyclonal antibody (Catalog # A08730-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP6 at approximately 40 kDa. The expected band size for SP6 is at 40 kDa.
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