GeneTexs affinity purified anti-SPANX-C antibody was used at 2.5 microg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human sperm and spermatids at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
![Western blot using GeneTexs Protein A purified anti-SPANX-C antibody shows detection of a band at ~17 kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye?800 conjugated goat anti-Rabbit IgG [H&L] was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated.](https://www.genetex.com/upload/website/prouct_img/normal/GTX48817/GTX48817_20160330_WB_w_23060823_289.webp "Western blot using GeneTexs Protein A purified anti-SPANX-C antibody shows detection of a band at ~17 kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells (left panel, arrowhead). VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000. IRDye?800 conjugated goat anti-Rabbit IgG [H&L] was used (left). IRDye is a trademark of LI-COR, Inc. Size estimation was made by comparison to prestained MW markers as indicated.")
GeneTexs affinity purified anti-SPANX-C antibody was used at 2.5 microg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of human sperm and spermatids at 60X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
SPANXC antibody
GTX48817
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSPANXC
Overview
- SupplierGeneTex
- Product NameSPANXC antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:5000. IHC-P: 1.25-2.5 microg/mL. ELISA: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration5 mg/ml
- ConjugateUnconjugated
- Gene ID64663
- Target nameSPANXC
- Target descriptionSPANX family member C
- Target synonymscancer/testis antigen 11.3; cancer/testis antigen family 11, member 3; cancer/testis-associated protein of 11 kD; cancer-testis-associated protein CTp11; CT11.3; CTp11; nuclear-associated protein SPAN-Xc; Nuclear-associated protein SPAN-Xe; SPANX-C; SPAN-Xc protein; SPANXE; SPANX-E; sperm protein associated with the nucleus on the X chromosome C; Sperm protein associated with the nucleus on the X chromosome E; sperm protein associated with the nucleus, X chromosome, family member C
- HostRabbit
- IsotypeIgG
- Protein IDQ9NY87
- Protein NameSperm protein associated with the nucleus on the X chromosome C
- Scientific DescriptionHuman Sperm Proteins Associated with the Nucleus on X-chromosome (SPANX) are relatively low molecular weight cytoplasmic proteins found in testis and sperm. Their expression in other tissues indicates malignancies. Family members are observed as proteins that range from 15 to 20 kDa.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203