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SPARCL1 Antibody

ORB255651
Biorbyt
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSPARCL1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    SPARCL1 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID8404
  • Target name
    SPARCL1
  • Target description
    SPARC like 1
  • Target synonyms
    MAST 9, MAST9, PIG33, SC1, SPARC-like protein 1, SPARC-like 1 (hevin), high endothelial venule protein, proliferation-inducing protein 33
  • Host
    Rabbit
  • Protein IDQ14515
  • Protein Name
    SPARC-like protein 1
  • Scientific Description
    SPARCL1 Antibody
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPARCL1 using anti-SPARCL1 antibody (A05726). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SHG-44 whole cell lysates, Lane 3: human MDA-MB-231 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse smooth muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARCL1 antigen affinity purified polyclonal antibody (Catalog # A05726) at 0.5mg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARCL1 at approximately 62KD. The expected band size for SPARCL1 is at 75KD.
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References
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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA268589(SPARCL1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
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WB analysis of various samples using GTX12308 SPARCL1 antibody. Lane 1 : rat lung tissue lysate Lane 2 : mouse lung tissue lysate Lane 3 : PANC cell lysate Lane 4 : A549 cell lysate Lane 5 : COLO320 cell lysate Lane 6 : MCF-7 cell lysate Lane 7 : HT1080 cell lysate
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