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SPARCL1 / Hevin Antibody (clone 01, Preservative Free)

LS-C820231
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
ReactivityHuman
TargetSPARCL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SPARCL1 / Hevin Antibody (clone 01, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA(Cap): This antibody will detect Human SPARCL1 / SPARC-like 1 in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Applications
    ELISA
  • Applications Supplier
    ELISA ELISA(Cap): This antibody will detect Human SPARCL1 / SPARC-like 1 in ELISA pair set. In a sandwich ELISA, it can be used as a capture antibody. Please Note: Optimal concentrations/dilutions should be determined by the end user.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    01
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8404
  • Target name
    SPARCL1
  • Target description
    SPARC like 1
  • Target synonyms
    MAST 9, MAST9, PIG33, SC1, SPARC-like protein 1, SPARC-like 1 (hevin), high endothelial venule protein, proliferation-inducing protein 33
  • Host
    Mouse
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPARCL1 using anti-SPARCL1 antibody (A05726). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SHG-44 whole cell lysates, Lane 3: human MDA-MB-231 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse smooth muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARCL1 antigen affinity purified polyclonal antibody (Catalog # A05726) at 0.5mg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARCL1 at approximately 62KD. The expected band size for SPARCL1 is at 75KD.
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