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Gel: 8%SDS-PAGE,Lysate: 40 ug, Primary antibody: CSB-PA186712(SPATA18 Antibody) at dilution 1/250 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 5 minutes
Gel: 8%SDS-PAGE,Lysate: 40 ug, Primary antibody: CSB-PA186712(SPATA18 Antibody) at dilution 1/250 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 5 minutes
Gel: 8%SDS-PAGE,Lysate: 40 ug, Primary antibody: CSB-PA186712(SPATA18 Antibody) at dilution 1/250 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 5 minutes

SPATA18 Antibody

CSB-PA186712
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSPATA18
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Overview

  • Supplier
    Cusabio
  • Product Name
    SPATA18 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID132671
  • Target name
    SPATA18
  • Target description
    spermatogenesis associated 18
  • Target synonyms
    Mieap, SPETEX1, mitochondria-eating protein, spermatogenesis associated 18 homolog, testis tissue sperm-binding protein Li 71n
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TC71
  • Protein Name
    Mitochondria-eating protein
  • Scientific Description
    SPATA18 (spermatogenesis associated 18), also known as Mieap or SPETEX1, is a 538 amino acid protein that is thought to play a role in cell differentiation during spermatogenesis, particularly during development from late elongate spematids to mature spermatozoa. Localizing to cytoplasm, SPATA18 is encoded by a gene that maps to human chromosome 4q12.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPATA18 using anti-SPATA18 antibody (A09906-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA18 antigen affinity purified polyclonal antibody (Catalog # A09906-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPATA18 at approximately 61 kDa. The expected band size for SPATA18 is at 61 kDa.
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