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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA928166(SPATA20 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA928166(SPATA20 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA928166(SPATA20 Antibody) at dilution 1/70, on the right is treated with synthetic peptide. (Original magnification: x200)

SPATA20 Antibody

CSB-PA928166
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSPATA20
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Overview

  • Supplier
    Cusabio
  • Product Name
    SPATA20 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64847
  • Target name
    SPATA20
  • Target description
    spermatogenesis associated 20
  • Target synonyms
    HEL-S-98, SSP411, Tisp78, spermatogenesis-associated protein 20, epididymis secretory protein Li 98, sperm protein SSP411, sperm-specific protein 411, transcript increased in spermiogenesis 78
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TB22
  • Protein Name
    Spermatogenesis-associated protein 20
  • Scientific Description
    The protein may play a role in fertility regulation. It were significantly upregulated in individual bile samples from Cholangiocarcinoma patients by Western blotting and Immunohistochemistry, relative to normal tissues.It acts as a potential serum diagnostic biomarker for Cholangiocarcinoma.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPATA20 using anti-SPATA20 antibody (A17005-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA20 antigen affinity purified polyclonal antibody (Catalog # A17005-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPATA20 at approximately 88 kDa. The expected band size for SPATA20 is at 88 kDa.
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