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ProductsBack/SPDEF Antibody - N-terminal region (ARP31841_T100)

SPDEF Antibody - N-terminal region (ARP31841_T100)

ARP31841_T100
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetSPDEF
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    SPDEF Antibody - N-terminal region (ARP31841_T100)
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  • Gene ID25803
  • Target name
    SPDEF
  • Target description
    SAM pointed domain containing ETS transcription factor
  • Target synonyms
    PDEF, bA375E1.3, SAM pointed domain-containing Ets transcription factor, prostate epithelium-specific Ets transcription factor, prostate-derived Ets factor, prostate-specific Ets
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSE/SPDEF using anti-PSE/SPDEF antibody (A04625-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSE/SPDEF antigen affinity purified polyclonal antibody (Catalog # A04625-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSE/SPDEF at approximately 44 kDa. The expected band size for PSE/SPDEF is at 38 kDa.
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