Bio-Connect

spectrin beta I (B-1) HRP

SC-374309HRP
Santa Cruz Biotechnology
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSPTB
200 ug
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    spectrin beta I (B-1) HRP
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    HRP
  • Gene ID6710
  • Target name
    SPTB
  • Target description
    spectrin beta, erythrocytic
  • Target synonyms
    beta-I spectrin; EL3; HS2; HSPTB1; membrane cytoskeletal protein; Sp beta; spectrin beta chain, erythrocyte; spectrin beta chain, erythrocytic; spectrin beta Tandil; SPH2
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

IHC-P analysis of mouse skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:20
Product group Antibodies
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetSPTB
  • SizePrice
Immunohistochemistry analysis in human skeletal muscle and liver tissues using HPA003398 antibody. Corresponding SPTB RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
TargetSPTB
  • SizePrice
Figure 1. Western blot analysis of beta 1 Spectrin/SPTB using anti-beta 1 Spectrin/SPTB antibody (A02392). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta 1 Spectrin/SPTB antigen affinity purified polyclonal antibody (Catalog # A02392) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta 1 Spectrin/SPTB at approximately 270 kDa. The expected band size for beta 1 Spectrin/SPTB is at 246 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetSPTB
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
TargetSPTB
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
TargetSPTB
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
TargetSPTB
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
TargetSPTB
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetSPTB
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetSPTB
  • SizePrice
Product group Antibodies
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetSPTB
  • SizePrice