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IHC-P analysis of mouse skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:20
IHC-P analysis of mouse skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:20
IHC-P analysis of mouse skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:20

Spectrin beta 1 antibody [4C3]

GTX22808
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSPTB
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Overview

  • Supplier
    GeneTex
  • Product Name
    Spectrin beta 1 antibody [4C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:100. ICC/IF: 1:10-1:200. IHC-P: 1:20-1:200. FACS: 0.5-2 microg/test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4C3
  • Conjugate
    Unconjugated
  • Gene ID6710
  • Target name
    SPTB
  • Target description
    spectrin beta, erythrocytic
  • Target synonyms
    beta-I spectrin; EL3; HS2; HSPTB1; membrane cytoskeletal protein; Sp beta; spectrin beta chain, erythrocyte; spectrin beta chain, erythrocytic; spectrin beta Tandil; SPH2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP11277
  • Protein Name
    Spectrin beta chain, erythrocytic
  • Scientific Description
    This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of beta 1 Spectrin/SPTB using anti-beta 1 Spectrin/SPTB antibody (A02392). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta 1 Spectrin/SPTB antigen affinity purified polyclonal antibody (Catalog # A02392) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta 1 Spectrin/SPTB at approximately 270 kDa. The expected band size for beta 1 Spectrin/SPTB is at 246 kDa.
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