IHC-P analysis of mouse skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:20
![IHC-P analysis of human prostate carcinoma tissue using GTX22808 Spectrin beta 1 antibody [4C3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX22808/GTX22808_1142_IHC-P_w_23060620_561.webp "IHC-P analysis of human prostate carcinoma tissue using GTX22808 Spectrin beta 1 antibody [4C3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:20")
![IHC-P analysis of human skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22808/GTX22808_1143_IHC-P_w_23060620_404.webp "IHC-P analysis of human skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:200")
![FACS analysis of Hela cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22808/GTX22808_151_FACS_w_23060620_707.webp "FACS analysis of Hela cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![FACS analysis of NIH/3T3 cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22808/GTX22808_152_FACS_w_23060620_116.webp "FACS analysis of NIH/3T3 cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue). Dilution : 0.5 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![FACS analysis of SH-SY5Y cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22808/GTX22808_153_FACS_w_23060620_801.webp "FACS analysis of SH-SY5Y cells using GTX22808 Spectrin beta 1 antibody [4C3] compared to an isotype control (blue). Dilution : 1 ug/test for 60 min at room temperature Fixation : 2% paraformaldehyde Permeabilization : 90% methanol and incubated for 10 minutes at room temperature")
![WB analysis of 25 ug of Hela cell lysates using GTX22808 Spectrin beta 1 antibody [4C3]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22808/GTX22808_1603_WB_w_23060620_160.webp "WB analysis of 25 ug of Hela cell lysates using GTX22808 Spectrin beta 1 antibody [4C3]. Dilution : 1:1000")
![ICC/IF analysis of A431 cells using GTX22808 Spectrin beta 1 antibody [4C3]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22808/GTX22808_485_ICC-IF_w_23060620_923.webp "ICC/IF analysis of A431 cells using GTX22808 Spectrin beta 1 antibody [4C3]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of Hela cells using GTX22808 Spectrin beta 1 antibody [4C3]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22808/GTX22808_486_ICC-IF_w_23060620_410.webp "ICC/IF analysis of Hela cells using GTX22808 Spectrin beta 1 antibody [4C3]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC")
IHC-P analysis of mouse skeletal muscle using GTX22808 Spectrin beta 1 antibody [4C3]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0) microwaved for 8-15 min Dilution : 1:20
Spectrin beta 1 antibody [4C3]
GTX22808
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSPTB
Overview
- SupplierGeneTex
- Product NameSpectrin beta 1 antibody [4C3]
- Delivery Days Customer9
- Antibody SpecificityThis antibody has been shown to specifically detect the two known alternatively spliced forms of spectrin, beta-1 epsilon-1, present in erythrocytes, and beta-1 epsilon-2, present in nerve and striated muscle cells. It does not cross-react with alpha-2 sp
- Application Supplier NoteWB: 1:100. ICC/IF: 1:10-1:200. IHC-P: 1:20-1:200. FACS: 0.5-2 microg/test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4C3
- ConjugateUnconjugated
- Gene ID6710
- Target nameSPTB
- Target descriptionspectrin beta, erythrocytic
- Target synonymsbeta-I spectrin; EL3; HS2; HSPTB1; membrane cytoskeletal protein; Sp beta; spectrin beta chain, erythrocyte; spectrin beta chain, erythrocytic; spectrin beta Tandil; SPH2
- HostMouse
- IsotypeIgG1
- Protein IDP11277
- Protein NameSpectrin beta chain, erythrocytic
- Scientific DescriptionThis locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203