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Western Blot analysis of Mouse brain and liver tissue using SPHK2 Polyclonal Antibody at dilution of 1:200
Western Blot analysis of Mouse brain and liver tissue using SPHK2 Polyclonal Antibody at dilution of 1:200
Western Blot analysis of Mouse brain and liver tissue using SPHK2 Polyclonal Antibody at dilution of 1:200

SPHK2 Polyclonal Antibody

E-AB-10958
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SPHK2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPHK2 using anti-SPHK2 antibody (A01382-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPHK2 antigen affinity purified polyclonal antibody (Catalog # A01382-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPHK2 at approximately 69KD. The expected band size for SPHK2 is at 69KD.
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