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ProductsBack/SPIDR / KIAA0146 Antibody (aa20-69)

SPIDR / KIAA0146 Antibody (aa20-69)

LS-C205889
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSPIDR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SPIDR / KIAA0146 Antibody (aa20-69)
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23514
  • Target name
    SPIDR
  • Target description
    scaffold protein involved in DNA repair
  • Target synonyms
    KIAA0146, ODG9, DNA repair-scaffolding protein, scaffolding protein involved in DNA repair
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPIDR using anti-SPIDR antibody (A04260-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPIDR antigen affinity purified polyclonal antibody (Catalog # A04260-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPIDR at approximately 95 kDa. The expected band size for SPIDR is at 100 kDa.
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