Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SPON1 Polyclonal Antibody, Unconjugated (bs-7520R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SPON1 Polyclonal Antibody, Unconjugated (bs-7520R) at 1:400 overnight at 4°C, DAB staining.
SPON1 Polyclonal Antibody
BS-7520R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
TargetSPON1
Overview
- SupplierBioss
- Product NameSPON1 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID10418
- Target nameSPON1
- Target descriptionspondin 1
- Target synonymsVSGP/F-spondin, f-spondin, spondin-1, spondin 1, (f-spondin) extracellular matrix protein, spondin 1, extracellular matrix protein, vascular smooth muscle cell growth-promoting factor
- HostRabbit
- IsotypeIgG
- Protein IDQ9HCB6
- Protein NameSpondin-1
- ReactivityBovine, Canine, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203
Datasheet
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