Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, SY5Y whole cell lysate, A549 whole cell lysate, HL-60 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, mouse brain tissue, rat brain tissue All lanes: SPPL3 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 19 kDa Observed band size: 43 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, U87 whole cell lysate, SY5Y whole cell lysate, A549 whole cell lysate, HL-60 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, mouse brain tissue, rat brain tissue All lanes: SPPL3 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 19 kDa Observed band size: 43 kDa
SPPL3 Antibody
CSB-PA848402LA01HU
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSPPL3
Overview
- SupplierCusabio
- Product NameSPPL3 Antibody
- Delivery Days Customer20
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID121665
- Target nameSPPL3
- Target descriptionsignal peptide peptidase like 3
- Target synonymsIMP2; intramembrane protease 2; MDHV1887; presenilin homologous protein 1; presenilin-like protein 4; PRO4332; PSH1; PSL4; signal peptide peptidase-like 3; SPP-like 3
- HostRabbit
- IsotypeIgG
- Protein IDQ8TCT6
- Protein NameSignal peptide peptidase-like 3
- Scientific DescriptionIntramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries (PubMed:16873890, PubMed:25354954, PubMed:25827571). Acts like a sheddase by mediating the proteolytic release and secretion of active site-containing ectodomains of glycan-modifiying glycosidase and glycosyltransferase enzymes such as MGAT5, B4GAT1 and B4GALT1 (PubMed:25354954, PubMed:25827571). Catalyzes the intramembrane cleavage of the envelope glycoprotein gp130 and/or the leader peptide gp18LP of the simian foamy virus independent of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis (PubMed:23132852). May also have the ability to serve as a shedding protease for subsequent intramembrane proteolysis by SPPL2A and SPPL2B of the envelope glycoprotein gp130 (PubMed:23132852). Plays a role in the regulation of cellular glycosylation processes (PubMed:25354954). Required to link T-cell antigen receptor (TCR) and calcineurin-NFAT signaling cascades in lymphocytes by promoting the association of STIM1 and ORAI1 during store-operated calcium entry (SOCE) in a protease-independent manner (PubMed:25384971).
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C
- UNSPSC12352203