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WB analysis of various sample lysates using GTX65548 SQRDL antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX65548 SQRDL antibody. Dilution : 1:3000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX65548 SQRDL antibody. Dilution : 1:3000 Loading : 25microg per lane

SQRDL antibody

GTX65548
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSQOR
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Overview

  • Supplier
    GeneTex
  • Product Name
    SQRDL antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID58472
  • Target name
    SQOR
  • Target description
    sulfide quinone oxidoreductase
  • Target synonyms
    CGI-44, PRO1975, SQR, SQRDL, sulfide:quinone oxidoreductase, mitochondrial, sulfide dehydrogenase like, sulfide quinone reductase-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y6N5
  • Protein Name
    Sulfide:quinone oxidoreductase, mitochondrial
  • Scientific Description
    The protein encoded by this gene may function in mitochondria to catalyze the conversion of sulfide to persulfides, thereby decreasing toxic concencrations of sulfide. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2012]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SQRDL/SQOR using anti-SQRDL/SQOR antibody (A31817). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQRDL/SQOR antigen affinity purified polyclonal antibody (Catalog # A31817) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SQRDL/SQOR at approximately 50 kDa. The expected band size for SQRDL/SQOR is at 50 kDa.
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