More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SQSTM1 Mouse Monoclonal Antibody [Clone ID: OTI4E2]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1 (1:2000).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1 (1:2000).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1 (1:2000).

SQSTM1 Mouse Monoclonal Antibody [Clone ID: OTI4E2]

CF502130
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSQSTM1
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    SQSTM1 Mouse Monoclonal Antibody [Clone ID: OTI4E2]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4E2
  • Gene ID8878
  • Target name
    SQSTM1
  • Target description
    sequestosome 1
  • Target synonyms
    A170, DMRV, EBIAP, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B, sequestosome-1, EBI3-associated protein of 60 kDa, EBI3-associated protein p60, autophagy receptor p62, oxidative stress induced like, phosphotyrosine independent ligand for the Lck SH2 domain p62, phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, ubiquitin-binding protein p62
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13501
  • Protein Name
    Sequestosome-1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SQSTM1 mouse monoclonal antibody, clone OTI4E2 (formerly 4E2)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
SQSTM1/p62 AntibodyCAB7758
ReactivityHuman
TargetSQSTM1
  • SizePrice
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA615696LA01HU at dilution of 1:100
Product group Antibodies
Cusabio
SQSTM1 AntibodyCSB-PA615696LA01HU
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSQSTM1
  • SizePrice
Anti-SQSTM1 Antibody
Product group Antibodies
Antibodies.com
Anti-SQSTM1 AntibodyA25004
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SQSTM1 AntibodyLS-C831297
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetSQSTM1
  • SizePrice
Product group Antibodies
MedChem Express
SQSTM1/p62 Antibody (YA669, Mouse)HY-P80518
TargetSQSTM1
  • SizePrice
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-SQSTM1 AntibodyHPA068843
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetSQSTM1
  • SizePrice
Product group Antibodies
Biomatik
Sqstm1 Polyclonal AntibodyCAC09268
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ELISA, ImmunoHistoChemistry
TargetSQSTM1
  • SizePrice
Figure 1. Western blot analysis of SQSTM1 using anti-SQSTM1 antibody (PB9444). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQSTM1 antigen affinity purified polyclonal antibody (Catalog # PB9444) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SQSTM1 at approximately 62 kDa. The expected band size for SQSTM1 is at 48 kDa.
Product group Antibodies
Boster Bio
Anti-SQSTM1/p62 Antibody Picoband(r)PB9444-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHamster, Human, Mouse, Rat
TargetSQSTM1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with SQSTM1 Polyclonal Antibody, Unconjugated (bs-2951R) at 1:200 overnight at 4°C, DAB staining.
Product group Antibodies
References
Bioss
SQSTM1/P62 Polyclonal AntibodyBS-2951R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetSQSTM1
  • SizePrice
Product group Antibodies
Biosynth
SQSTM1 antibody10R-5910
  • SizePrice