![ICC/IF analysis of PFA-fixed HeLa cells using GTX00955 SQSTM1 / P62 antibody [GT1193]. Orange : Primary antibody Blue : DAPI Dilution : 1:100 ICC/IF analysis of PFA-fixed HeLa cells using GTX00955 SQSTM1 / P62 antibody [GT1193]. Orange : Primary antibody Blue : DAPI Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00955/GTX00955_20200327_ICC-IF_26_w_23053121_109.webp)
ICC/IF analysis of PFA-fixed HeLa cells using GTX00955 SQSTM1 / P62 antibody [GT1193]. Orange : Primary antibody Blue : DAPI Dilution : 1:100
SQSTM1 / P62 antibody [GT1193]
GTX00955
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSQSTM1
Overview
- SupplierGeneTex
- Product NameSQSTM1 / P62 antibody [GT1193]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. IP: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1193
- ConjugateUnconjugated
- Gene ID8878
- Target nameSQSTM1
- Target descriptionsequestosome 1
- Target synonymsA170, DMRV, EBIAP, FTDALS3, NADGP, OSIL, PDB3, ZIP3, p60, p62, p62B, sequestosome-1, EBI3-associated protein of 60 kDa, EBI3-associated protein p60, autophagy receptor p62, oxidative stress induced like, phosphotyrosine independent ligand for the Lck SH2 domain p62, phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, ubiquitin-binding protein p62
- HostRabbit
- IsotypeIgG
- Protein IDQ13501
- Protein NameSequestosome-1
- Scientific DescriptionThis gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zhang Z, Yi J, Xie B, et al. Parkin, as a Regulator, Participates in Arsenic Trioxide-Triggered Mitophagy in HeLa Cells. Curr Issues Mol Biol. 2022,44(6):2759-2771. doi: 10.3390/cimb44060189Read this paper
![ICC/IF analysis of PFA-fixed L929 cells using GTX00955 SQSTM1 / P62 antibody [GT1193]. Orange : Primary antibody Blue : DAPI Dilution : 1:100 ICC/IF analysis of PFA-fixed L929 cells using GTX00955 SQSTM1 / P62 antibody [GT1193]. Orange : Primary antibody Blue : DAPI Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00955/GTX00955_20200327_ICC-IF_27_w_23053121_669.webp)
![IHC-P analysis of Rat spleen tissue section using GTX00955 SQSTM1 / P62 antibody [GT1193]. Dilution : 1:100 IHC-P analysis of Rat spleen tissue section using GTX00955 SQSTM1 / P62 antibody [GT1193]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00955/GTX00955_20200327_IHC-P_24_w_23053121_173.webp)
![IHC-P analysis of human lung cancer tissue section using GTX00955 SQSTM1 / P62 antibody [GT1193]. Dilution : 1:100 IHC-P analysis of human lung cancer tissue section using GTX00955 SQSTM1 / P62 antibody [GT1193]. Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX00955/GTX00955_20200327_IHC-P_25_w_23053121_151.webp)
![WB analysis of various samples using GTX00955 SQSTM1 / P62 antibody [GT1193]. Dilution : 1:1000 Loading : 25microg WB analysis of various samples using GTX00955 SQSTM1 / P62 antibody [GT1193]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00955/GTX00955_20200327_IP_1_w_23053121_275.webp)
![WB analysis of wild-type (WT) and SQSTM1 / p62 knockout (KO) 293T cell extracts using GTX00955 SQSTM1 / P62 antibody [GT1193]. Dilution : 1:1000 Laoding : 25microg WB analysis of wild-type (WT) and SQSTM1 / p62 knockout (KO) 293T cell extracts using GTX00955 SQSTM1 / P62 antibody [GT1193]. Dilution : 1:1000 Laoding : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00955/GTX00955_20200327_WB_1_w_23053121_471.webp)
![IP analysis of HeLa cell lysate using GTX00955 SQSTM1 / P62 antibody [GT1193]. Antibody amount : 3microg / 300microg lysate Dilution : 1:1000 IP analysis of HeLa cell lysate using GTX00955 SQSTM1 / P62 antibody [GT1193]. Antibody amount : 3microg / 300microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX00955/GTX00955_20210323_IP_11_w_23053121_551.webp)
![Untreated (-) and treated (+) HepG2 whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [GT1193] (GTX00955) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Untreated (-) and treated (+) HepG2 whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [GT1193] (GTX00955) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00955/GTX00955_40000000180_20200306_WB_treatment_Thapsigargin_w_23053121_144.webp)



