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ProductsBack/SR (1H4) Alexa Fluor(r) 594

SR (1H4) Alexa Fluor(r) 594

SC-13509AF594
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSRSF1
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    SR (1H4) Alexa Fluor(r) 594
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 594
  • Gene ID6426
  • Target name
    SRSF1
  • Target description
    serine and arginine rich splicing factor 1
  • Target synonyms
    ASF, NEDFBA, SF2, SF2p33, SFRS1, SRp30a, serine/arginine-rich splicing factor 1, SR splicing factor 1, alternative-splicing factor 1, pre-mRNA-splicing factor SF2, P33 subunit, splicing factor 2, splicing factor, arginine/serine-rich, 30-KD, A
  • Host
    Mouse
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SF2/SRSF1 using anti-SF2/SRSF1 antibody (PB9404). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SF2/SRSF1 antigen affinity purified polyclonal antibody (Catalog # PB9404) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SF2/SRSF1 at approximately 28 kDa. The expected band size for SF2/SRSF1 is at 28 kDa.
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