![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with SRC3 antibody [N1N2], N-term (GTX113869) diluted at 1:2000 and competitor's antibody (SC-7216) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product. Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with SRC3 antibody [N1N2], N-term (GTX113869) diluted at 1:2000 and competitor's antibody (SC-7216) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113869/GTX113869_40150_20221020_WB_competitor_watermark_22102723_648.webp)
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membranes were blotted with SRC3 antibody [N1N2], N-term (GTX113869) diluted at 1:2000 and competitor's antibody (SC-7216) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
SRC3 antibody [N1N2], N-term
GTX113869
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNCOA3
Overview
- SupplierGeneTex
- Product NameSRC3 antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8202
- Target nameNCOA3
- Target descriptionnuclear receptor coactivator 3
- Target synonymsACTR, AIB-1, AIB1, CAGH16, CTG26, KAT13B, RAC3, SRC-3, SRC3, TNRC14, TNRC16, TRAM-1, bHLHe42, pCIP, nuclear receptor coactivator 3, CBP-interacting protein, amplified in breast cancer 1 protein, class E basic helix-loop-helix protein 42, receptor-associated coactivator 3, steroid receptor coactivator protein 3, thyroid hormone receptor activator molecule 1
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y6Q9
- Protein NameNuclear receptor coactivator 3
- Scientific DescriptionThe protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161


![Immunofluorescence analysis of methanol-fixed HeLa, using SRC3 antibody [N1N2], N-term (GTX113869) at 1:200 dilution. Immunofluorescence analysis of methanol-fixed HeLa, using SRC3 antibody [N1N2], N-term (GTX113869) at 1:200 dilution.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113869/GTX113869_40150_IFA_w_23060501_569.webp)






![WB analysis of truncated Trx-NCOA3 recombinant protein (1) andtruncated NCOA3 (aa1-200)-hIgGFc transfected CHOK1 cell lysate (2) using GTX83144 SRC3 antibody [2C11B12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83144/GTX83144_20170912_WB_w_23061322_344.webp)
![WB analysis of MCF-7 cell extract using GTX22782 SRC3 antibody [AX15.3].](https://www.genetex.com/upload/website/prouct_img/normal/GTX22782/GTX22782_1590_WB_w_23060620_366.webp)