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Western Blot analysis of HepG2 cells using p140Cap Polyclonal Antibody
Western Blot analysis of HepG2 cells using p140Cap Polyclonal Antibody
Western Blot analysis of HepG2 cells using p140Cap Polyclonal Antibody

SRCIN1 Antibody

CSB-PA003616
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSRCIN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SRCIN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80725
  • Target name
    SRCIN1
  • Target description
    SRC kinase signaling inhibitor 1
  • Target synonyms
    P140, SNIP, SRC kinase signaling inhibitor 1, P130Cas-associated protein, SNAP-25-interacting protein, SNAP25-interacting protein, p140Cap
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C0H9
  • Protein Name
    SRC kinase signaling inhibitor 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SRCIN1 using anti-SRCIN1 antibody (A08110-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRCIN1 antigen affinity purified polyclonal antibody (Catalog # A08110-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRCIN1 at approximately 140KD. The expected band size for SRCIN1 is at 140KD.
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