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Western Blot analysis of Jurkat cells using SRF Polyclonal Antibody
Western Blot analysis of Jurkat cells using SRF Polyclonal Antibody
Western Blot analysis of Jurkat cells using SRF Polyclonal Antibody

SRF Antibody

CSB-PA004158
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSRF
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Overview

  • Supplier
    Cusabio
  • Product Name
    SRF Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6722
  • Target name
    SRF
  • Target description
    serum response factor
  • Target synonyms
    MCM1, serum response factor, c-fos serum response element-binding transcription factor, minichromosome maintenance 1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11831
  • Protein Name
    Serum response factor
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Serum Response Factor/SRF using anti-Serum Response Factor/SRF antibody (A00557-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human Caco-1 whole cell lysates, Lane 7: rat C6 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Response Factor/SRF antigen affinity purified polyclonal antibody (Catalog # A00557-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Serum Response Factor/SRF at approximately 67KD. The expected band size for Serum Response Factor/SRF is at 67KD.
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